https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:50272 Wed 12 Jul 2023 14:11:21 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:12630 Wed 11 Apr 2018 10:07:15 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:46692 Tue 29 Nov 2022 09:42:38 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:52779 Tue 14 Nov 2023 14:21:49 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:55499 Tue 04 Jun 2024 20:56:10 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:48156 Thu 09 Mar 2023 09:44:46 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:39279 max) ranged from 0.29 to 2.18 x 10⁻² mM g⁻¹ soil h⁻¹ and declined by 42.30%–71.01% under PTH stress. The Michaelis constant (K_m) values ranged between 2.90 and 14.17 x 10⁻² mM and exhibited three forms including unchanged, increased (38.16–242.65%) and decreased (13.41–39.23%) when exposed to PTH. Based on the changes in two kinetic parameters, the inhibition of PTH on FDA–H was classified as three types, i.e., noncompetitive, linear mixed and uncompetitive inhibition. The competitive inhibition constant (K_ic) and noncompetitive constant (K_iu) ranged from 0.064 to 0.447 mM and 0.209 to 0.723 mM, respectively, which were larger than the Km in values. The catalytic efficiency (V_max/K_m) of FDA–H is a sensitive integrated parameter to evaluate the PTH toxicity due to the higher inhibition ratio than the V_max. The PTH toxicity to FDA–H decreased with increase of soil organic matter and total nitrogen contents. This implied that the PTH toxicity could be alleviated by an increasing content of soil organic matter due to its buffering capacity to PTH. Besides, soils with a higher content of total nitrogen could provide stable environment for FDA–H to maintain its functionality under PTH pollution. Thus, the results of this study have great implications to the risk assessment of parathion in soils.]]> Thu 02 Jun 2022 15:12:26 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:53429 Mon 27 Nov 2023 11:40:44 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:39536 Mon 08 Aug 2022 11:27:21 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:39691 Fri 17 Jun 2022 16:07:06 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:32983 max) and Michaelis constant (K_m) in unpolluted soils were 0.012-0.267 mM h^-1 and 1.34-3.79 mM respectively. The competitive inhibition constant (K_ic) was 0.17-0.70 mM, which was lower than K_m, suggesting higher enzyme affinity for As than for substrate. The ecological doses, ED₁₀ and ED₅₀ (concentration of As that results in 10% and 50% inhibition on enzyme parameter) for inhibition of catalytic efficiency (V_max/K_m) were lower than those for inhibition of enzyme activity at different substrate concentrations. This suggests that the integrated kinetic parameter, catalytic efficiency is substrate concentration independent and more sensitive to As than ALP activity. Thus, catalytic efficiency was proposed as a more reliable indicator than ALP activity for risk assessment of As pollution.]]> Fri 17 Aug 2018 15:44:20 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:52970 Fri 03 Nov 2023 15:30:26 AEDT ]]>